Fiche publication
Date publication
juin 2019
Journal
Biopreservation and biobanking
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DALSTEIN Véronique
,
Dr BOULAGNON-ROMBI Camille
,
Dr NASRI Saviz
Tous les auteurs :
Dudez O, Dalstein V, Kanagaratnam L, Nasri S, Coquelet C, Fichel C, Bouland N, Lemaire E, Diebold MD, Marchal-Bressenot A, Boulagnon-Rombi C
Lien Pubmed
Résumé
Oncology research projects are highly dependent on the quality of tumor samples stored in the biobank. Microscopic control is important to ensure the quality of the frozen sample (Does the sample correspond to tumor tissue? Does the sample contain a sufficient number of tumor cells for molecular analysis?). The aim of this study was to evaluate the value of the mirror image method in quality control of colonic adenocarcinoma samples stored in a tumor bank. Microscopic concordance for the differentiation grade, malignant and normal cell percentages, necrosis, mucinous component, and ulceration was assessed on 82 colon adenocarcinoma banked samples and their paired, formalin-fixed, paraffin-embedded mirror controls. Molecular concordance for status was evaluated in 76 of these 82 cases. Morphological correspondence between frozen and mirror samples was good for the mucinous component (intraclass correlation coefficient [ICC] = 0.81), moderate for differentiation (Cohen's kappa coefficient [] = 0.67), fair for malignant cells (ICC = 0.44), and poor for ulceration ( = 0.08), normal tissue (ICC = 0.36), and necrosis (ICC = 0.13) percentages. Molecular correspondence for status was almost perfect (95% correspondence, = 0.88) between frozen and mirror samples. In conclusion, the mirror sample method is not a good alternative for microscopic and molecular control of frozen colonic adenocarcinoma samples.
Mots clés
RNA quality, biobanking, colorectal cancer, mirror sample, pathology
Référence
Biopreserv Biobank. 2019 Jun 24;:
