Fiche publication
Date publication
juin 2016
Journal
Biomacromolecules
Auteurs
Membres identifiés du Cancéropôle Est :
Dr LAVALLE Philippe
,
Dr VOEGEL Jean-Claude
,
Dr FRANCIUS Grégory
,
Pr SCHAAF Pierre
Tous les auteurs :
Knopf-Marques H, Singh S, Htwe SS, Wolfova L, Buffa R, Bacharouche J, Francius G, Voegel JC, Schaaf P, Ghaemmaghami AM, Vrana NE, Lavalle P
Lien Pubmed
Résumé
This study aims to design an optimal polyelectrolyte multilayer film of poly-l-lysine (PLL) and hyaluronic acid (HA) as an anti-inflammatory cytokine release system in order to decrease the implant failure due to any immune reactions. The chemical modification of the HA with aldehyde moieties allows self-cross-linking of the film and an improvement in the mechanical properties of the film. The cross-linking of the film and the release of immunomodulatory cytokine (IL-4) stimulate the differentiation of primary human monocytes seeded on the films into pro-healing macrophages phenotype. This induces the production of anti-inflammatory cytokines (IL1-RA and CCL18) and the decrease of pro-inflammatory cytokines secreted (IL-12, TNF-α, and IL-1β). Moreover, we demonstrate that cross-linking PLL/HA film using HA-aldehyde is already effective by itself to limit inflammatory processes. Finally, this functionalized self-cross-linked PLL/HA-aldehyde films constitutes an innovative and efficient candidate for immunomodulation of any kind of implants of various architecture and properties.
Référence
Biomacromolecules. 2016 Jun;17(6):2189-98
