Fiche publication
Date publication
juillet 2019
Journal
Journal of biophotonics
Auteurs
Membres identifiés du Cancéropôle Est :
Pr SOCKALINGUM Ganesh
,
Pr THIEFIN Gérard
Tous les auteurs :
Lovergne L, Lovergne J, Bouzy P, Untereiner V, Offroy M, Garnotel R, Thiéfin G, Baker MJ, Sockalingum GD
Lien Pubmed
Résumé
Infrared spectroscopy is a rapid, easy-to-operate, label-free and therefore cost-effective technique. Many studies performed on biofluids (e.g. serum, plasma, urine, sputum, bile, cerebrospinal fluid) have demonstrated its promising application as a clinical diagnostic tool. Given all these characteristics, infrared spectroscopy appears to be an ideal candidate to be implemented into the clinics. However, before considering its translation, a clear effort is needed to standardise protocols for biofluid spectroscopic analysis. To reach this goal, careful investigations to identify and track errors that can occur during the pre-analytical phase is a crucial step. Here, we report for the first time, results of investigations into pre-analytical factors that can affect the quality of the spectral data acquired on serum and plasma, such as the impact of long-term freezing time storage of samples as well as the month- to-month reproducibility of the spectroscopic analysis. The spectral data discrimination has revealed to be majorly impacted by a residual water content variation in serum and plasma dried samples. This article is protected by copyright. All rights reserved.
Mots clés
FTIR spectroscopy, biofluids, plasma, pre-analytical requirements, serum, standardisation, water interference correction
Référence
J Biophotonics. 2019 Jul 5;:e201900177
