Lipid Emulsions Containing Medium Chain Triacylglycerols Blunt Bradykinin-Induced Endothelium-Dependent Relaxation in Porcine Coronary Artery Rings.

Fiche publication

Date publication

mars 2017

Journal

Lipids

Auteurs

Membres identifiés du Cancéropôle Est :
Pr SCHINI-KERTH Valérie , Dr AUGER Cyril

Tous les auteurs :
Amissi S, Boisramé-Helms J, Burban M, Rashid SK, León-González AJ, Auger C, Toti F, Meziani F, Schini-Kerth VB

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/28044232

Résumé

Lipid emulsions for parenteral nutrition are used to provide calories and essential fatty acids for patients. They have been associated with hypertriglyceridemia, hypercholesterolemia, and metabolic stress, which may promote the development of endothelial dysfunction in patients. The aim of the present study was to determine whether five different industrial lipid emulsions may affect the endothelial function of coronary arteries. Porcine coronary artery rings were incubated with lipid emulsions 0.5, 1, or 2% (v/v) for 30 min before the determination of vascular reactivity in organ chambers and the level of oxidative stress using electron paramagnetic resonance. Incubation of coronary artery rings with either Lipidem, Medialipid containing long- and medium-chain triacylglycerols (LCT/MCT), or SMOFlipid containing LCT, MCT, omega-9, and -3, significantly reduced the bradykinin-induced endothelium-dependent relaxation, affecting both the nitric oxide (NO) and endothelium-dependent hyperpolarization (EDH) components, whereas, Intralipid containing LCT (soybean oil) and ClinOleic containing LCT (soybean and olive oil) did not have such an effect. The endothelial dysfunction induced by Lipidem was significantly improved by indomethacin, a cyclooxygenase (COX) inhibitor, inhibitors of oxidative stress (N-acetylcysteine, superoxide dismutase, catalase) and transition metal chelating agents (neocuproine, tetrathiomolybdate, deferoxamine and L-histidine). Lipidem significantly increased the arterial level of oxidative stress. The present findings indicate that lipid emulsions containing LCT/MCT induce endothelial dysfunction in coronary artery rings by blunting both NO- and EDH-mediated relaxations. The Lipidem-induced endothelial dysfunction is associated with increased vascular oxidative stress and the formation of COX-derived vasoconstrictor prostanoids.