Fiche publication
Date publication
août 1992
Journal
The Biochemical journal
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GUEANT Jean-Louis
,
Pr SCHOHN Hervé
Tous les auteurs :
Schohn H, Guéant JL, Leheup B, Saunier M, Grignon G, Nicolas JP
Lien Pubmed
Résumé
Intrinsic factor receptor activity was observed in mucosal homogenates from whole small intestine and colon of 10-19-week fetuses, whereas it was only detected in the distal part of the small intestine of a 25-week fetus. The receptor-intrinsic factor-cobalamin complex was eluted into the void-volume position when ileum mucosal extract was assayed for receptor activity by gel filtration after incubation with either fetal gastric extract or human gastric juice. The intrinsic-factor-binding capacity of intestinal mucosal extracts ranged from 2.6 to 30.5 fmol/mg and was correlated with the gestational age of six fetuses. The dissociation constant of the receptor for the intrinsic factor-cobalamin complex was estimated at 0.24-0.36 nM at pH 7.4. In conclusion, intrinsic-factor-receptor activity was detected in the whole intestine in 10-19-week fetuses, whereas it was only present in the distal ileum at the end of fetal development.
Mots clés
Chromatography, Gel, Colon, embryology, Embryonic and Fetal Development, physiology, Fetus, Gestational Age, Humans, Intestinal Mucosa, embryology, Intestine, Small, embryology, Intrinsic Factor, metabolism, Kinetics, Radioligand Assay, Receptors, Cell Surface, isolation & purification, Receptors, Peptide
Référence
Biochem. J.. 1992 Aug;286 ( Pt 1):153-6
