Fiche publication
Date publication
juillet 2019
Journal
Nature
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BAUMERT Thomas
,
Pr PESSAUX Patrick
Tous les auteurs :
Aizarani N, Saviano A, Sagar,Mailly L, Durand S, Herman JS, Pessaux P, Baumert TF, Grün D
Lien Pubmed
Résumé
The human liver is an essential multifunctional organ. The incidence of liver diseases is rising and there are limited treatment options. However, the cellular composition of the liver remains poorly understood. Here we performed single-cell RNA sequencing of about 10,000 cells from normal liver tissue from nine human donors to construct a human liver cell atlas. Our analysis identified previously unknown subtypes of endothelial cells, Kupffer cells, and hepatocytes, with transcriptome-wide zonation of some of these populations. We show that the EPCAM population is heterogeneous, comprising hepatocyte-biased and cholangiocyte populations as well as a TROP2 progenitor population with strong potential to form bipotent liver organoids. As a proof-of-principle, we used our atlas to unravel the phenotypic changes that occur in hepatocellular carcinoma cells and in human hepatocytes and liver endothelial cells engrafted into a mouse liver. Our human liver cell atlas provides a powerful resource to enable the discovery of previously unknown cell types in normal and diseased livers.
Référence
Nature. 2019 Jul 10;:
