ABCE1: A special factor that orchestrates translation at the crossroad between recycling and initiation.

Fiche publication

Date publication

octobre 2017

Journal

RNA biology

Auteurs

Membres identifiés du Cancéropôle Est :
Dr SIMONETTI Angelita

Tous les auteurs :
Mancera-Martínez E, Brito Querido J, Valasek LS, Simonetti A, Hashem Y

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/28498001

Résumé

For many years initiation and termination of mRNA translation have been studied separately. However, a direct link between these 2 isolated stages has been suggested by the fact that some initiation factors also control termination and can even promote ribosome recycling; i.e. the last stage where post-terminating 80S ribosomes are split to start a new round of initiation. Notably, it is now firmly established that, among other factors, ribosomal recycling critically requires the NTPase ABCE1. However, several earlier reports have proposed that ABCE1 also somehow participates in the initiation complex assembly. Based on an extended analysis of our recently published late-stage 48S initiation complex from rabbit, here we provide new mechanistic insights into this putative role of ABCE1 in initiation. This point of view represents the first structural evidence in which the regulatory role of the recycling factor ABCE1 in initiation is discussed and establishes a corner stone for elucidating the interplay between ABCE1 and several initiation factors during the transit from ribosomal recycling to formation of the elongation competent 80S initiation complex.