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Date publication
novembre 2016
Journal
The Journal of organic chemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MELY Yves
Tous les auteurs :
Barthes NP, Gavvala K, Bonhomme D, Dabert-Gay AS, Debayle D, Mély Y, Michel BY, Burger A
Lien Pubmed
Résumé
We report the synthesis and site-specific incorporation in oligodeoxynucleotides (ODNs) of an emissive deoxyuridine analog electronically conjugated on its C5-position with a 3-methoxychromone moiety acting as a fluorophore. When incorporated in ODNs, this fluorescent deoxyuridine analog exhibits remarkable photostability and good quantum yields. This deoxyuridine analog also displays a mega-Stokes shift, which allows for its use as an efficient donor for FRET-based studies when paired with the yellow emissive indocarbocyanine Cy3 acceptor.
Référence
J. Org. Chem.. 2016 Nov;81(22):10733-10741
