Multimodal characterization of acid-pretreated poplar reveals spectral and structural parameters strongly correlate with saccharification.

Fiche publication

Date publication

août 2019

Journal

Bioresource technology

Auteurs

Membres identifiés du Cancéropôle Est :
Dr TERRYN Christine

Tous les auteurs :
Zoghlami A, Refahi Y, Terryn C, Paës G

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/31454737

Résumé

Lignocellulose biomass can be transformed into sustainable chemicals, materials and energy but its natural recalcitrance requires the use of pretreatment to enhance subsequent catalytic steps. Dilute acid pretreatment is one of the most common and efficient ones, however its impact has not yet been investigated simultaneously at nano- and cellular-scales. Poplar samples have been pretreated by dilute acid at different controlled severities, then characterized by combined structural and spectral techniques (scanning electron microscopy, confocal microscopy, autofluorescence, fluorescence lifetime, Raman). Results show that pretreatment favours lignin depolymerization until severity of 2.4-2.5 while at severity of 2.7 lignin seems to repolymerize as revealed by broadening of autofluorescence spectrum and strong decrease in fluorescence lifetime. Importantly, both nano-scale and cellular-scale markers can predict hydrolysis yield of pretreated samples, highlighting some connections in the multiscale recalcitrance of lignocellulose.