Fiche publication
Date publication
octobre 2016
Journal
Journal of proteomics
Auteurs
Membres identifiés du Cancéropôle Est :
Mr HAMMANN Philippe
Tous les auteurs :
Poirier I, Kuhn L, Demortière A, Mirvaux B, Hammann P, Chicher J, Caplat C, Pallud M, Bertrand M
Lien Pubmed
Résumé
In the marine environment, bacteria from estuarine and coastal sediments are among the first targets of nanoparticle pollution; it is therefore relevant to improve the knowledge of interactions between bacteria and nanoparticles. In this work, the response of the marine bacterium Pseudomonas fluorescens BA3SM1 to CdSe nanocrystals (CdSe NPs) of 3nm (NP3) and 8nm (NP8) in diameter was evaluated through microscopic, physiological, biochemical and proteomic approaches. Transmission electron microscopy images showed that NP3 were able to penetrate the bacteria, while NP8 were highly concentrated around the cells, embedded in large exopolysaccharides. In our experimental conditions, both CdSe NP sizes induced a decrease in respiration during the stationary growth phase, while only NP8 caused growth retardation and a decrease in pyoverdine production. Proteomic analyses highlighted that the strain responded to CdSe NP toxicity by inducing various defence mechanisms such as cell aggregation, extracellular CdSe NP sequestration, effective protection against oxidative stress, modifications of envelope organization and properties, and cadmium export. In addition, BA3SM1 presented a biosorption capacity of 1.6×10(16)NP3/g dry weight and 1.7×10(15)NP8/g dry weight. This strain therefore appears as a promising agent for NP bioremediation processes. Proteomic data are available via ProteomeXchange with identifier PXD004012.
Mots clés
Biodegradation, Environmental, Cadmium Compounds, pharmacokinetics, Ecosystem, Industrial Waste, Metal Nanoparticles, chemistry, Particle Size, Proteomics, Pseudomonas fluorescens, drug effects, Selenium Compounds, pharmacokinetics, Water Pollutants, Chemical, toxicity
Référence
J Proteomics. 2016 10 4;148:213-27
