Metastatic Tumor Cells Exploit Their Adhesion Repertoire to Counteract Shear Forces during Intravascular Arrest.

Fiche publication

Date publication

septembre 2019

Journal

Cell reports

Auteurs

Membres identifiés du Cancéropôle Est :
Dr GOETZ Jacky , Dr LEFEBVRE Olivier , Dr HARLEPP Sébastien , Dr OSMANI Naël

Tous les auteurs :
Osmani N, Follain G, García León MJ, Lefebvre O, Busnelli I, Larnicol A, Harlepp S, Goetz JG

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/31484062

Résumé

Cancer metastasis is a process whereby a primary tumor spreads to distant organs. We have demonstrated previously that blood flow controls the intravascular arrest of circulating tumor cells (CTCs) through stable adhesion to endothelial cells. We now aim to define the contribution of cell adhesion potential and identify adhesion receptors at play. Early arrest is mediated by the formation of weak adhesion, depending on CD44 and integrin αvβ3. Stabilization of this arrest uses integrin α5β1-dependent adhesions with higher adhesion strength, which allows CTCs to stop in vascular regions with lower shear forces. Moreover, blood flow favors luminal deposition of fibronectin on endothelial cells, an integrin α5β1 ligand. Finally, we show that only receptors involved in stable adhesion are required for subsequent extravasation and metastasis. In conclusion, we identified the molecular partners that are sequentially exploited by CTCs to arrest and extravasate in vascular regions with permissive flow regimes.