Fiche publication
Date publication
mai 2016
Journal
Nutrients
Auteurs
Membres identifiés du Cancéropôle Est :
Pr DELMAS Dominique
,
Dr LANCON Allan
,
Dr LIMAGNE Emeric
Tous les auteurs :
Limagne E, Lançon A, Delmas D, Cherkaoui-Malki M, Latruffe N
Lien Pubmed
Résumé
State of the art. Osteoarthritis (OA) is a chronic articular disease characterized by cartilage degradation and osteophyte formation. OA physiopathology is multifactorial and involves mechanical and hereditary factors. So far, there is neither preventive medicine to delay cartilage breakdown nor curative treatment. Objectives. To investigate pro-inflammatory paracrine interactions between human primary chondrocytes and macrophages following interleukin-1-β (IL-1β) treatment; to evaluate the molecular mechanism responsible for the inhibitory effect of resveratrol. Results. The activation of NF-κB in chondrocytes by IL-1β induced IL-6 secretion. The latter will then activate STAT3 protein in macrophages. Moreover, STAT3 was able to positively regulate IL-6 secretion, as confirmed by the doubling level of IL-6 in the coculture compared to macrophage monoculture. These experiments confirm the usefulness of the coculture model in the inflammatory arthritis-linked process as a closer biological situation to the synovial joint than separated chondrocytes and macrophages. Il also demonstrated the presence of an inflammatory amplification loop induced by IL-1β. Resveratrol showed a strong inhibitory effect on the pro-inflammatory marker secretion. The decrease of IL-6 secretion is dependent on the NFκB inhibition in the chondrocytes. Such reduction of the IL-6 level can limit STAT3 activation in the macrophages, leading to the interruption of the inflammatory amplification loop. Conclusion. These results increase our understanding of the anti-inflammatory actions of resveratrol and open new potential approaches to prevent and treat osteoarthritis.
Mots clés
Anti-Inflammatory Agents, Non-Steroidal, administration & dosage, Biomarkers, Cells, Cultured, Chondrocytes, drug effects, Coculture Techniques, Humans, Inflammation, metabolism, Interleukin-1beta, pharmacology, Macrophages, drug effects, Stilbenes, administration & dosage, Time Factors
Référence
Nutrients. 2016 May;8(5):
