Fiche publication
Date publication
septembre 2019
Journal
Nature communications
Auteurs
Membres identifiés du Cancéropôle Est :
Dr HAMICHE Ali
,
Dr KLAHOLZ Bruno
Tous les auteurs :
Andronov L, Ouararhni K, Stoll I, Klaholz BP, Hamiche A
Lien Pubmed
Résumé
CENP-A is an essential histone H3 variant that epigenetically marks the centromeric region of chromosomes. Here we show that CENP-A nucleosomes form characteristic clusters during the G1 phase of the cell cycle. 2D and 3D super-resolution microscopy and segmentation analysis reveal that these clusters encompass a globular rosette-like structure, which evolves into a more compact structure in late G1. The rosette-like clusters contain numerous CENP-A molecules and form a large cellular structure of ∼250-300 nm diameter with remarkably similar shapes for each centromere. Co-localization analysis shows that HJURP, the CENP-A chaperone, is located in the center of the rosette and serves as a nucleation point. The discovery of an HJURP-mediated CENP-A nucleation in human cells and its structural description provide important insights into the mechanism of CENP-A deposition and the organization of CENP-A chromatin in the centromeric region.
Référence
Nat Commun. 2019 Sep 30;10(1):4436
