Fiche publication
Date publication
août 2016
Journal
Scientific reports
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MONCHAUD David
Tous les auteurs :
Laguerre A, Wong JM, Monchaud D
Lien Pubmed
Résumé
Guanine-rich DNA or RNA sequences can fold into higher-order, four-stranded structures termed quadruplexes that are suspected to play pivotal roles in cellular mechanisms including the control of the genome integrity and gene expression. However, the biological relevance of quadruplexes is still a matter of debate owing to the paucity of unbiased evidences of their existence in cells. Recent reports on quadruplex-specific antibodies and small-molecule fluorescent probes help dispel reservations and accumulating evidences now pointing towards the cellular relevance of quadruplexes. To better assess and comprehend their biology, developing new versatile tools to detect both DNA and RNA quadruplexes in cells is essential. We report here a smart fluorescent probe that allows for the simple detection of quadruplexes thanks to an uncommon spectroscopic mechanism known as the red-edge effect (REE). We demonstrate that this effect could open avenues to greatly enhance the ability to visualize both DNA and RNA quadruplexes in human cells, using simple protocols and fluorescence detection facilities.
Mots clés
DNA, chemistry, Fluorescent Dyes, chemistry, G-Quadruplexes, Guanine, chemistry, Humans, MCF-7 Cells, Microscopy, Confocal, RNA, chemistry, Spectrometry, Fluorescence
Référence
Sci Rep. 2016 08 18;6:32141
