Fiche publication
Date publication
août 2016
Journal
Biological trace element research
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BREZILLON Stéphane
Tous les auteurs :
Bagheri Varzaneh M, Rahmani H, Jahanian R, Mahdavi AH, Perreau C, Perrot G, Brézillon S, Maquart FX
Lien Pubmed
Résumé
The objective of the present study was to investigate the effects of different levels of copper (as supplemental copper-methionine) on ascites incidence and matrix metalloproteinase-2 (MMP-2) changes in the lungs of cold-stressed broilers. For this purpose, 480 1-day-old Ross 308 broiler chickens were randomly assigned to six treatments. Treatments consisted of two ambient temperatures (thermoneutral and cold stress) each combined with 0, 100, and 200 mg supplemental copper/kg as copper-methionine in a 2 × 3 factorial arrangement in a completely randomized design with four replicates. Ascites was diagnosed based on abdominal and pericardial fluid accumulation at 45 days of age. Fourty-eight broilers were killed at 38 and 45 days of age, and their lungs were collected for biological analysis. Results showed that MMP-2 increased in the lungs of ascitic broilers and that copper-methionine supplementation significantly reduced MMP-2 in cold-stressed broiler chickens. Treatments did not affect tissue inhibitor of metalloproteinase-2 (TIMP-2) at 38 and 45 days of age, and no difference was observed between 100 and 200 mg/kg copper-methionine treatments. In conclusion, copper-methionine at higher than conventional levels of supplementation decreased ascites incidence in low temperature through reduced MMP-2 concentration. Further research is warranted to investigate the effect of copper on MMP-2 concentrations in other tissues with high oxygen demand.
Mots clés
Animals, Chickens, metabolism, Copper, administration & dosage, Dietary Supplements, Disease Models, Animal, Dose-Response Relationship, Drug, Hypertension, Pulmonary, drug therapy, Lung, drug effects, Matrix Metalloproteinase 2, genetics, Methionine, administration & dosage, RNA, Messenger, genetics
Référence
Biol Trace Elem Res. 2016 Aug;172(2):504-10
