Fiche publication
Date publication
juillet 2016
Journal
Angewandte Chemie (International ed. in English)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr SPECHT Alexandre
Tous les auteurs :
Fichte MA, Weyel XM, Junek S, Schäfer F, Herbivo C, Goeldner M, Specht A, Wachtveitl J, Heckel A
Lien Pubmed
Résumé
We successfully introduced two-photon-sensitive photolabile groups ([7-(diethylamino)coumarin-4-yl]methyl and p-dialkylaminonitrobiphenyl) into DNA strands and demonstrated their suitability for three-dimensional photorelease. To visualize the uncaging, we used a fluorescence readout based on double-strand displacement in a hydrogel and in neurons. Orthogonal two-photon uncaging of the two cages is possible, thus enabling complex scenarios of three-dimensional control of hybridization with light.
Mots clés
DNA, hydrogels, photochemistry, two-photon uncaging, wavelength selectivity
Référence
Angew. Chem. Int. Ed. Engl.. 2016 07 25;55(31):8948-52
