Photolabile glutamate protecting group with high one- and two-photon uncaging efficiencies.

Date publication

mai 2008

Journal

Chembiochem : a European journal of chemical biology

Auteurs

Membres identifiés du Cancéropôle Est :
Dr SPECHT Alexandre

Tous les auteurs :
Gug S, Charon S, Specht A, Alarcon K, Ogden D, Zietz B, Léonard J, Haacke S, Bolze F, Nicoud JF, Goeldner M

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/18386275

Résumé

A pi-extended [2-(2-nitrophenyl)propoxy]carbonyl (NPPOC) derivative has been prepared as an efficient UV and near-IR photolabile protecting group for glutamate. This glutamate cage compound exhibits efficient photorelease upon one-photon excitation (epsilonPhi=990 M(-1) cm(-1) at 315 nm). In addition, it also shows efficient photorelease in activation of glutamate receptors in electrophysiological recordings. Combined with a high two-photon uncaging cross-section (deltaPhi=0.45 GM at 800 nm), its overall properties make this new cage-3-(2-propyl)-4'-methoxy-4-nitrobiphenyl (PMNB)-for glutamate a very promising tool for two-photon neuronal studies.

Mots clés

Electrophysiology, Glutamic Acid, chemical synthesis, Hippocampus, metabolism, Molecular Structure, Patch-Clamp Techniques, Photochemistry, Photons, Spectrophotometry, Tissue Culture Techniques

Référence

Chembiochem. 2008 May 23;9(8):1303-7