Fiche publication
Date publication
juillet 2016
Journal
PLoS genetics
Auteurs
Membres identifiés du Cancéropôle Est :
Dr HAMICHE Ali
Tous les auteurs :
Charles Richard JL, Shukla MS, Menoni H, Ouararhni K, Lone IN, Roulland Y, Papin C, Ben Simon E, Kundu T, Hamiche A, Angelov D, Dimitrov S
Lien Pubmed
Résumé
FACT, in addition to its role in transcription, is likely implicated in both transcription-coupled nucleotide excision repair and DNA double strand break repair. Here, we present evidence that FACT could be directly involved in Base Excision Repair and elucidate the chromatin remodeling mechanisms of FACT during BER. We found that, upon oxidative stress, FACT is released from transcription related protein complexes to get associated with repair proteins and chromatin remodelers from the SWI/SNF family. We also showed the rapid recruitment of FACT to the site of damage, coincident with the glycosylase OGG1, upon the local generation of oxidized DNA. Interestingly, FACT facilitates uracil-DNA glycosylase in the removal of uracil from nucleosomal DNA thanks to an enhancement in the remodeling activity of RSC. This discloses a novel property of FACT wherein it has a co-remodeling activity and strongly enhances the remodeling capacity of the chromatin remodelers. Altogether, our data suggest that FACT may acts in concert with RSC to facilitate excision of DNA lesions during the initial step of BER.
Mots clés
Animals, Chromatin, genetics, Chromatin Assembly and Disassembly, genetics, Chromosomal Proteins, Non-Histone, genetics, DNA Damage, genetics, DNA Repair, genetics, DNA-Binding Proteins, biosynthesis, HeLa Cells, High Mobility Group Proteins, biosynthesis, Histones, genetics, Humans, Nucleosomes, genetics, Oxidative Stress, genetics, Saccharomyces cerevisiae, genetics, Saccharomyces cerevisiae Proteins, genetics, Transcription Factors, genetics, Transcription, Genetic, Transcriptional Elongation Factors, biosynthesis, Uracil, metabolism, Xenopus laevis
Référence
PLoS Genet.. 2016 07;12(7):e1006221
