Fiche publication
Date publication
mai 2019
Journal
The journal of physical chemistry letters
Auteurs
Membres identifiés du Cancéropôle Est :
Dr KLYMCHENKO Andrey
Tous les auteurs :
Ashoka AH, Ashokkumar P, Kovtun YP, Klymchenko AS
Lien Pubmed
Résumé
Can polarity-sensitive fluorescent dyes monitor the response of live cells to fundamental stress conditions, such as deprivation from nutrition and oxidative stress? To address this question, we developed a push-pull dioxaborine probe (DXB-NIR) for biomembranes and lipid droplets featuring strong solvatochromism in the far-red to near-infrared region, high fluorescence brightness, photostability, and two-photon absorption cross section, reaching 13800 GM at 930 nm. In model membranes, DXB-NIR exhibits unprecedented 80 nm shift between liquid ordered and disordered membrane phases, allowing robust imaging of separated membrane microdomains. Two-color imaging of live cells with DXB-NIR enables polarity mapping in plasma membranes, endoplasmic reticulum, and lipid droplets, which reveals that starvation and oxidative stress produce an increase in the local polarity, and this change is different for each of the studied cell compartments. Thus, by pushing the limits of existing solvatochromic dyes, we introduce a concept of polarity mapping for monitoring the response of cells to stress.
Mots clés
Boron Compounds, chemistry, Fluorescence, Fluorescent Dyes, chemistry, HeLa Cells, Humans, Infrared Rays, Lipids, chemistry, Molecular Structure, Optical Imaging, Oxidative Stress, Particle Size, Spectrometry, Fluorescence
Référence
J Phys Chem Lett. 2019 May 16;10(10):2414-2421
