Fiche publication
Date publication
avril 2018
Journal
Journal of the American Chemical Society
Auteurs
Membres identifiés du Cancéropôle Est :
Dr KLYMCHENKO Andrey
Tous les auteurs :
Collot M, Fam TK, Ashokkumar P, Faklaris O, Galli T, Danglot L, Klymchenko AS
Lien Pubmed
Résumé
Lipid droplets (LDs) are intracellular lipid-rich organelles that regulate the storage of neutral lipids and were recently found to be involved in many physiological processes, metabolic disorders, and diseases including obesity, diabetes, and cancers. Herein we present a family of new fluorogenic merocyanine fluorophores based on an indolenine moiety and a dioxaborine barbiturate derivative. These so-called StatoMerocyanines (SMCy) fluoresce from yellow to the near-infrared (NIR) in oil with an impressive fluorescence enhancement compared to aqueous media. Additionally, SMCy display remarkably high molar extinction coefficients (up to 390 000 M cm) and high quantum yield values (up to 100%). All the members of this new family specifically stain the LDs in live cells with very low background noise. Unlike Nile Red, a well-known lipid droplet marker, SMCy dyes possess narrow absorption and emission bands in the visible, thus allowing multicolor imaging. SMCy proved to be compatible with fixation and led to high-quality 3D images of lipid droplets in cells and tissues. Their high brightness allowed efficient tissue imaging of adipocytes and circulating LDs. Moreover their remarkably high two-photon absorption cross-section, especially SMCy5.5 (up to 13 300 GM), as well as their capacity to efficiently fluoresce in the NIR region led to two-photon multicolor tissue imaging (liver). Taking advantage of the available color palette, lipid droplet exchange between cells was tracked and imaged, thus demonstrating intercellular communication.
Mots clés
Adipose Tissue, cytology, Animals, Benzopyrans, chemistry, Fluorescent Dyes, chemistry, Humans, Indoles, chemistry, KB Cells, Lipid Droplets, ultrastructure, Liver, ultrastructure, Mice, Mice, Inbred C57BL, Microscopy, Fluorescence, methods, Models, Molecular, Optical Imaging, methods
Référence
J. Am. Chem. Soc.. 2018 04 25;140(16):5401-5411
