Ca-NIR: a ratiometric near-infrared calcium probe based on a dihydroxanthene-hemicyanine fluorophore.

Date publication

juin 2017

Journal

Chemical communications (Cambridge, England)

Auteurs

Membres identifiés du Cancéropôle Est :
Dr KLYMCHENKO Andrey

Tous les auteurs :
Collot M, Ponsot F, Klymchenko AS

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/28530289

Résumé

Fluorescent calcium probes are essential tools for studying the fluctuation of calcium ions in cells. Herein, we developed Ca-NIR, the first ratiometric calcium probe emitting in the near infrared region. This probe arose from the fusion of a BAPTA chelator and a dihydroxanthene-hemicyanine fluorophore. It is efficiently excited with common 630-640 nm lasers and displays two distinct emission bands depending on the calcium concentration (K = ∼8 μM). The physicochemical and spectroscopic properties of Ca-NIR allowed for ratiometric imaging of calcium distribution in live cells.

Référence

Chem. Commun. (Camb.). 2017 Jun 1;53(45):6117-6120