SMART Digest™ compared with pellet digestion for analysis of human immunoglobulin G1 in rat serum by liquid chromatography tandem mass spectrometry.

Date publication

mai 2016

Journal

Analytical biochemistry

Auteurs

Membres identifiés du Cancéropôle Est :
Dr CIANFERANI Sarah

Tous les auteurs :
Lanshoeft C, Heudi O, Cianférani S

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/26893107

Résumé

The newly developed SMART Digest™ kit was applied for the sample preparation of human immunoglobulin G1 (hIgG1) in rat serum prior to qualitative and quantitative analyses by liquid chromatography tandem mass spectrometry (LC-MS/MS). The sequence coverages obtained for the light and heavy chains of hIgG1A were 50 and 76%, respectively. The calibration curve was linear from 1.00 to 1000 μg/ml for three of four generic peptides. Overall, the SMART Digest™ kit resulted in similar quantitative data (linearity, sensitivity, accuracy, and precision) compared with the pellet digestion protocol. However, the SMART Digest™ required only 2 h of sample preparation with fewer reagents.

Mots clés

Amino Acid Sequence, Animals, Chromatography, Liquid, Humans, Immunoglobulin G, analysis, Limit of Detection, Rats, Tandem Mass Spectrometry

Référence

Anal. Biochem.. 2016 May;501:23-5