Reverse transcriptase multiplex ligation-dependent probe amplification in endomyocardial biopsies for the diagnosis of cardiac allograft rejection.

Date publication

novembre 2019

Journal

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation

Auteurs

Membres identifiés du Cancéropôle Est :
Pr CHENARD Marie-Pierre

Tous les auteurs :
Adam N, Coutance G, Viailly PJ, Drieux F, Ruminy P, Sater AA, Toquet C, Rouvier P, François A, Chenard MP, Epailly E, Guillemain R, Pattier S, Gay A, Varnous S, Taupin JL, Rabant M, Loupy A, Bruneval P, Duong Van Huyen JP

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/31822443

Résumé

Molecular biology has emerged as a potential companion to histology for the diagnosis of rejection after heart transplantation. Reverse transcriptase multiplex ligation-dependent probe amplification (RT-MLPA) is a technique of targeted gene expression analysis suitable for formalin-fixed paraffin-embedded (FFPE) biopsies. Our aim was to assess RT-MLPA for the diagnosis of allograft rejection in heart transplantation.

Mots clés

Allograft Rejection, class prediction analysis, endomyocardial biopsy, heart transplantation, histopathology, molecular biology

Référence

J. Heart Lung Transplant.. 2019 Nov 26;: