Fiche publication
Date publication
janvier 2020
Journal
Platelets
Auteurs
Membres identifiés du Cancéropôle Est :
Dr ERHARDT Mathieu
,
Dr GACHET Christian
Tous les auteurs :
Scandola C, Erhardt M, Rinckel JY, Proamer F, Gachet C, Eckly A
Lien Pubmed
Résumé
Electron microscopy (EM) has a long history in megakaryocyte (MK) cellular biology. This chapter shows how the electron microscope, since its first appearance almost 90 years ago, has occupied center stage in the studies of MK morphology and function. It describes some of the more productive EM techniques that have shaped our understanding of the physiology of thrombopoiesis. These include the standard transmission and scanning EM techniques as well as the new imaging methods, correlative microscopy and volume EM which provide information on the 3D organization of MKs on different scales: single organelles, whole cells and tissues. For each technique, we list the advantages and limitations, the resolution that can be achieved, the technical difficulties and the applications in MK biology.
Mots clés
Bone marrow, correlative light electron microscopy, electron microscopy, megakaryocytes
Référence
Platelets. 2020 Jan 5;:1-10
