Fiche publication
Date publication
janvier 2020
Journal
Genetics
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MENDOZA Manuel
Tous les auteurs :
Di Stefano M, Di Giovanni F, Pozharskaia V, Gomar-Alba M, Baù D, Carey LB, Marti-Renom MA, Mendoza M
Lien Pubmed
Résumé
The three-dimensional organization of chromosomes can influence transcription. However, the frequency and magnitude of these effects remain debated. To determine how changes in chromosome positioning affect transcription across thousands of genes with minimal perturbation, we characterized nuclear organization and global gene expression in budding yeast containing chromosome fusions. We used computational modeling and single-cell imaging to determine chromosome position and integrated these data with genome-wide transcriptional profiles from RNA sequencing. We find that chromosome fusions dramatically alter 3D nuclear organization without leading to strong genome-wide changes in transcription. However, we observe a mild but significant and reproducible increase in the expression of genes displaced away from the periphery. The increase in transcription is inversely proportional to the propensity of a given locus to be at the nuclear periphery; for example, a 10% decrease in the propensity of a gene to reside at the NE is accompanied by a 10% increase in gene expression. Modeling suggests that this is due to both deletion of telomeres and to displacement of genes relative to the nuclear periphery. These data suggest that basal transcriptional activity is sensitive to radial changes on gene position, and provide insight into the functional relevance of budding yeast chromosome-level three-dimensional organization in gene expression.
Mots clés
budding yeast, computational modelling, gene expression, nuclear organization, single cell imaging
Référence
Genetics. 2020 Jan 6;:
