Double compartmented and hybrid implant outfitted with well-organized 3D stem cells for osteochondral regenerative nanomedicine.

Date publication

septembre 2015

Auteurs

Membres identifiés du Cancéropôle Est :
Dr BENKIRANE-JESSEL Nadia

Tous les auteurs :
Keller L, Wagner Q, Schwinte P, Benkirane-Jessel N

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/26377156

Résumé

AIM: Articular cartilage repair remains challenging, because most clinical failures are due to the lack of subchondral bone regeneration. We report an innovative approach improving cartilage repair by regenerating a robust subchondral bone, supporting articular cartilage. MATERIALS & METHODS: We developed a compartmented living implant containing triple-3D structure: stem cells as microtissues for embryonic endochondral development mimic, nanofibrous collagen to enhance mineralization for subchondral bone and alginate hydrogel for cartilage regeneration. RESULTS & CONCLUSION: This system mimics the natural gradient of the osteochondral unit, using only one kind of stem cell, targeting their ability to express specific bone or cartilage proteins. Mineralization gradient of articular cartilage and the natural 'glue' between subchondral bone and cartilage were reproduced in vitro.

Référence

Nanomedicine (Lond). 2015 Sep;10(18):2833-45