Fiche publication
Date publication
février 2016
Journal
Nature communications
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BIRCK Catherine
,
Dr SERAPHIN Bertrand
Tous les auteurs :
Stupfler B, Birck C, Séraphin B, Mauxion F
Lien Pubmed
Résumé
While BTG2 plays an important role in cellular differentiation and cancer, its precise molecular function remains unclear. BTG2 interacts with CAF1 deadenylase through its APRO domain, a defining feature of BTG/Tob factors. Our previous experiments revealed that expression of BTG2 promoted mRNA poly(A) tail shortening through an undefined mechanism. Here we report that the APRO domain of BTG2 interacts directly with the first RRM domain of the poly(A)-binding protein PABPC1. Moreover, PABPC1 RRM and BTG2 APRO domains are sufficient to stimulate CAF1 deadenylase activity in vitro in the absence of other CCR4-NOT complex subunits. Our results unravel thus the mechanism by which BTG2 stimulates mRNA deadenylation, demonstrating its direct role in poly(A) tail length control. Importantly, we also show that the interaction of BTG2 with the first RRM domain of PABPC1 is required for BTG2 to control cell proliferation.
Mots clés
Blotting, Western, Cell Line, Tumor, Cell Proliferation, HEK293 Cells, Humans, Immediate-Early Proteins, metabolism, Immunoprecipitation, In Vitro Techniques, Poly(A)-Binding Protein I, metabolism, Protein Structure, Tertiary, RNA, Messenger, metabolism, Transcription Factors, metabolism, Tumor Suppressor Proteins, metabolism
Référence
Nat Commun. 2016 Feb;7:10811
