Fiche publication
Date publication
février 2020
Journal
Scientific reports
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BENKIRANE-JESSEL Nadia
Tous les auteurs :
Bugueno IM, Zobairi El-Ghazouani F, Batool F, El Itawi H, Anglès-Cano E, Benkirane-Jessel N, Toti F, Huck O
Lien Pubmed
Résumé
A link between periodontitis and atherothrombosis has been highlighted. The aim of this study was to determine the influence of Porphyromonas gingivalis on endothelial microvesicles (EMV) shedding and their contribution to endothelial inflammation. Endothelial cells (EC) were infected with P. gingivalis (MOI = 100) for 24 h. EMV were isolated and their concentration was evaluated by prothrombinase assay. EMV were significantly increased in comparison with EMV shedded by unstimulated cells. While EMV from untreated EC had no effect, whereas, the proportion of apoptotic EC was increased by 30 nM EMV and viability was decreased down to 25%, a value elicited by P. gingivalis alone. Moreover, high concentration of EMV (30 nM) induced a pro-inflammatory and pro-oxidative cell response including up-regulation of TNF-α, IL-6 and IL-8 as well as an altered expression of iNOS and eNOS at both mRNA and protein level. An increase of VCAM-1 and ICAM-1 mRNA expression (4.5 folds and 3 folds respectively (p < 0.05 vs untreated) was also observed after EMV (30 nM) stimulation whereas P. gingivalis infection was less effective, suggesting a specific triggering by EMV. Kinasome analysis demonstrated the specific effect induced by EMV on main pro-inflammatory pathways including JNK/AKT and STAT. EMV are effective pro-inflammatory effectors that may have detrimental effect on vascular homeostasis and should be considered as potential autocrine and paracrine effectors involved in the link between periodontitis and atherothrombosis.
Référence
Sci Rep. 2020 Feb 4;10(1):1778
