Fiche publication
Date publication
janvier 2016
Journal
Frontiers in cellular and infection microbiology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GANGLOFF Sophie
,
Dr VELARD Frédéric
Tous les auteurs :
Josse J, Guillaume C, Bour C, Lemaire F, Mongaret C, Draux F, Velard F, Gangloff SC
Lien Pubmed
Résumé
Staphylococcus aureus is one of the most frequently involved pathogens in bacterial infections such as skin abscess, pneumonia, endocarditis, osteomyelitis, and implant-associated infection. As for bone homeostasis, it is partly altered during infections by S. aureus by the induction of various responses from osteoblasts, which are the bone-forming cells responsible for extracellular matrix synthesis and its mineralization. Nevertheless, bone-forming cells are a heterogeneous population with different stages of maturation and the impact of the latter on their responses toward bacteria remains unclear. We describe the impact of S. aureus on two populations of human primary bone-forming cells (HPBCs) which have distinct maturation characteristics in both acute and persistent models of interaction. Cell maturation did not influence the internalization and survival of S. aureus inside bone-forming cells or the cell death related to the infection. By studying the expression of chemokines, cytokines, and osteoclastogenic regulators by HPBCs, we observed different profiles of chemokine expression according to the degree of cell maturation. However, there was no statistical difference in the amounts of proteins released by both populations in the presence of S. aureus compared to the non-infected counterparts. Our findings show that cell maturation does not impact the behavior of HPBCs infected with S. aureus and suggest that the role of bone-forming cells may not be pivotal for the inflammatory response in osteomyelitis.
Mots clés
Calcification, Physiologic, Cell Death, Cell Differentiation, drug effects, Cell Line, Cells, Cultured, Chemokines, metabolism, Collagen Type I, Cytokines, metabolism, Dexamethasone, pharmacology, Host-Pathogen Interactions, Humans, Microscopy, Electron, Scanning, Osteoblasts, metabolism, Osteocalcin, Osteomyelitis, metabolism, Staphylococcal Infections, complications, Staphylococcus aureus, pathogenicity
Référence
Front Cell Infect Microbiol. 2016 ;6:64
