Fiche publication
Date publication
octobre 1995
Journal
Infection and immunity
Auteurs
Membres identifiés du Cancéropôle Est :
Pr CRIBIER Bernard
Tous les auteurs :
Prévost G, Cribier B, Couppié P, Petiau P, Supersac G, Finck-Barbançon V, Monteil H, Piemont Y
Lien Pubmed
Résumé
Staphylococcus aureus ATCC 49775 produces three proteins recognized by affinity-purified antibodies against the S component of Panton-Valentine leucocidin (LukS-PV) and two proteins recognized by affinity-purified antibodies against the F component of this toxin (LukF-PV). Purification of these proteins and cloning of the corresponding genes provided evidence for the presence of two loci. The first one, encoding Panton-Valentine leucocidin, consisted of two cotranscribed open reading frames, lukS-PV and lukF-PV, coding the class S and the class F components, respectively. The second one coded for a gamma-hemolysin and consisted of two transcription units, the first one encoding an HlgA-like protein, a class S component, and the second one encoding two cotranscribed open reading frames identical to HlgC and HlgB, class S and class F components, respectively, from gamma-hemolysin from the reference strain Smith 5R. It appears that the Panton-Valentine leucocidin from S. aureus ATCC 49775 (V8 strain) should not be confused with leucocidin from ATCC 27733 (another isolate of V8 strain), which had 95% identity with HlgC and HlgB from gamma-hemolysin. The cosecretion of these five proteins led to six possible synergistic combinations between F and S components. Two of these combinations (LukS-PV-LukF-PV and HlgA-LukF-PV) had dermonecrotic activity on rabbit skin, and all six were leukocytolytic on glass-adsorbed leukocytes. Only three were hemolytic on rabbit erythrocytes, the two gamma-hemolysin combinations and the combination LukF-PV-HlgA.
Mots clés
Amino Acid Sequence, Animals, Bacterial Proteins, Bacterial Toxins, chemistry, Base Sequence, Cloning, Molecular, Hemolysin Proteins, genetics, Humans, Leukocidins, chemistry, Molecular Sequence Data, Rabbits, Sheep, Staphylococcus aureus, pathogenicity, Transcription, Genetic
Référence
Infect. Immun.. 1995 Oct;63(10):4121-9
