Reconstitution of Functionally Active Thermus thermophilus 30S Ribosomal Subunit from Ribosomal 16S RNA and Ribosomal Proteins.

Date publication

janvier 2016

Journal

Methods in molecular biology (Clifton, N.J.)

Auteurs

Membres identifiés du Cancéropôle Est :
Dr YUSUPOV Marat , Dr YUSUPOVA Gulnara

Tous les auteurs :
Agalarov S, Yusupov M, Yusupova G

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/26227051

Résumé

In vitro reconstitution systems of ribosomal subunits from free ribosomal RNA and ribosomal proteins are helpful tool for studies on the structure, function and assembly of ribosome. Using this system mutant or modified ribosomal proteins or ribosomal RNA can be incorporated into ribosomal subunits for studying ribosome structure and function. Developing the protocol for reconstitution of 30S subunits from an extreme thermophilic bacterium Thermus thermophilus can be beneficial especially for structural studies, as proteins and nucleic acids from this organism are very stable and crystallize easier than those from mesophilic organisms.

Mots clés

Centrifugation, Density Gradient, Crystallization, Nucleic Acid Conformation, Nucleic Acids, chemistry, Poly U, Polyamines, chemistry, RNA, chemistry, RNA, Bacterial, genetics, RNA, Ribosomal, genetics, RNA, Ribosomal, 16S, chemistry, RNA, Transfer, chemistry, Ribosomal Proteins, chemistry, Ribosome Subunits, Small, Bacterial, chemistry, Ribosomes, chemistry, Thermus thermophilus, chemistry

Référence

Methods Mol. Biol.. 2016 ;1320:303-14