Fiche publication
Date publication
juin 2017
Journal
Trends in microbiology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr HOCQUET Didier
Tous les auteurs :
Sauget M, Valot B, Bertrand X, Hocquet D
Lien Pubmed
Résumé
Bacterial typing is crucial to tackle the spread of bacterial pathogens but current methods are time-consuming and costly. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been recently integrated into the microbiology laboratory workflow for a quick and low-cost microbial species identification. Independent research groups have successfully redirected the original function of this technology from their primary purpose to discriminate subgroups within pathogen species. However, identical bacterial subgroups could be identified by unrelated peaks by independent methods, thus limiting their robustness and exportability. We propose several guidelines that could improve the performance of MALDI-TOF MS-based typing methods for use as a first-line epidemiological tool.
Mots clés
Bacteria, Escherichia coli, MALDI-TOF MS, Staphylococcus aureus, Typing
Référence
Trends Microbiol.. 2017 06;25(6):447-455
