Fiche publication
Date publication
septembre 2004
Journal
Genes & development
Auteurs
Membres identifiés du Cancéropôle Est :
Pr CHAMBON Pierre
,
Mr LEROUGE Thierry
Tous les auteurs :
Cammas F, Herzog M, Lerouge T, Chambon P, Losson R
Lien Pubmed
Résumé
The transcriptional intermediary factor 1beta (TIF1beta) is a corepressor for KRAB-domain-containing zinc finger proteins and is believed to play essential roles in cell physiology by regulating chromatin organization at specific loci through association with chromatin remodeling and histone-modifying activities and recruitment of heterochromatin protein 1 (HP1) proteins. In this study, we have engineered a modified embryonal carcinoma F9 cell line (TIF1beta(HP1box/-)) expressing a mutated TIF1beta protein (TIF1beta(HP1box)) unable to interact with HP1 proteins. Phenotypic analysis of TIF1beta(HP1box/-) and TIF1beta(+/-) cells shows that TIF1beta-HP1 interaction is not required for differentiation of F9 cells into primitive endoderm-like (PrE) cells on retinoic acid (RA) treatment but is essential for further differentiation into parietal endoderm-like (PE) cells on addition of cAMP and for differentiation into visceral endoderm-like cells on treatment of vesicles with RA. Complementation experiments reveal that TIF1beta-HP1 interaction is essential only during a short window of time within early differentiating PrE cells to establish a selective transmittable competence to terminally differentiate on further cAMP inducing signal. Moreover, the expression of three endoderm-specific genes, GATA6, HNF4, and Dab2, is down-regulated in TIF1beta(HP1box/-) cells compared with wild-type cells during PrE differentiation. Collectively, these data demonstrate that the interaction between TIF1beta and HP1 proteins is essential for progression through differentiation by regulating the expression of endoderm differentiation master players.
Mots clés
Adaptor Proteins, Signal Transducing, Adaptor Proteins, Vesicular Transport, metabolism, Blotting, Western, Cell Differentiation, genetics, Chromosomal Proteins, Non-Histone, metabolism, Cyclic AMP, DNA Primers, DNA-Binding Proteins, genetics, Endoderm, physiology, Fluorescent Antibody Technique, GATA6 Transcription Factor, Gene Expression Regulation, Genes, Tumor Suppressor, Genetic Complementation Test, Genetic Vectors, genetics, Hepatocyte Nuclear Factor 4, Humans, Microscopy, Confocal, Mutagenesis, Site-Directed, Mutation, genetics, Phosphoproteins, metabolism, Repressor Proteins, genetics, Reverse Transcriptase Polymerase Chain Reaction, Transcription Factors, metabolism, Tretinoin, Tumor Cells, Cultured, Tumor Suppressor Proteins
Référence
Genes Dev.. 2004 Sep;18(17):2147-60
