Fiche publication
Date publication
mars 1990
Journal
The Journal of biological chemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Pr CHAMBON Pierre
,
Dr GRONEMEYER Hinrich
,
Mr LEROUGE Thierry
Tous les auteurs :
Jeltsch JM, Turcotte B, Garnier JM, Lerouge T, Krozowski Z, Gronemeyer H, Chambon P
Lien Pubmed
Résumé
The structure of the 42-kilobase (kb) long chicken progesterone receptor (cPR) gene and of all six transcripts that are detectable on Northern blots was determined. The first of 8 exons encodes the N-terminal region A/B which is highly divergent among different species and contains a constitutive transcription activation function. The DNA (DBD)- and hormone-binding domains (HBD) are assembled from 2 and 5 exons, respectively, with the individual "zinc fingers" of the DBD encoded by separate exons. In addition to the previously described 4.5-kb cPR mRNA species, alternative polyadenylation, splicing variation, and "5'-truncation" lead to the generation of 5 further mRNAs. Most importantly, this 5'-truncation produces, by an as yet unidentified mechanism, an abundant transcript which encodes form A but not form B of cPR. Lack of splicing at the exon 2 splice-donor and polyadenylation due to a signal site in the second intron generates a previously undetected 3.4-kb mRNA species. The corresponding cDNA was sequenced in its entirety and shown to encode only region A/B and the N-terminal "finger" of the DBD. Alternative polyadenylation upstream of the signal site for the 4.5-kb mRNA is responsible for the appearance of a 3.3-kb mRNA. The longest cPR mRNA (8.2 kb) originates from a transcription termination point more than 3 kb downstream of the 4.5-kb mRNA 3'-end. Finally, the primary sequence of more than 2 kb upstream sequences of the cPR gene, containing several consensus hexamer progestin/glucocorticoid receptor-binding sites (PRE/GRE and putative Sp1 binding motifs, is discussed.
Mots clés
Amino Acid Sequence, Animals, Base Sequence, Blotting, Northern, Cell Nucleus, metabolism, Chickens, DNA, genetics, Exons, Genes, Genetic Variation, Molecular Sequence Data, RNA Splicing, RNA, Messenger, genetics, Receptors, Progesterone, genetics, Restriction Mapping, Sequence Homology, Nucleic Acid, Transcription, Genetic
Référence
J. Biol. Chem.. 1990 Mar;265(7):3967-74
