Fiche publication
Date publication
mars 2020
Journal
The EMBO journal
Auteurs
Membres identifiés du Cancéropôle Est :
Dr GIANGRANDE Angela
,
Dr MOLINA Nacho
,
Dr CATTENOZ Pierre
Tous les auteurs :
Cattenoz PB, Sakr R, Pavlidaki A, Delaporte C, Riba A, Molina N, Hariharan N, Mukherjee T, Giangrande A
Lien Pubmed
Résumé
Immune cells provide defense against non-self and have recently been shown to also play key roles in diverse processes such as development, metabolism, and tumor progression. The heterogeneity of Drosophila immune cells (hemocytes) remains an open question. Using bulk RNA sequencing, we find that the hemocytes display distinct features in the embryo, a closed and rapidly developing system, compared to the larva, which is exposed to environmental and metabolic challenges. Through single-cell RNA sequencing, we identify fourteen hemocyte clusters present in unchallenged larvae and associated with distinct processes, e.g., proliferation, phagocytosis, metabolic homeostasis, and humoral response. Finally, we characterize the changes occurring in the hemocyte clusters upon wasp infestation, which triggers the differentiation of a novel hemocyte type, the lamellocyte. This first molecular atlas of hemocytes provides insights and paves the way to study the biology of the Drosophila immune cells in physiological and pathological conditions.
Mots clés
Drosophila melanogaster , immune cells, single-cell RNA-seq, wasp infestation
Référence
EMBO J.. 2020 Mar 12;:e104486
