Deciphering the role of insertion sequences in the evolution of bacterial epidemic pathogens with software.

Fiche publication

Date publication

mars 2020

Journal

Microbial genomics

Auteurs

Membres identifiés du Cancéropôle Est :
Pr HOCQUET Didier

Tous les auteurs :
Couchoud C, Bertrand X, Valot B, Hocquet D

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/32213253

Résumé

Next-generation sequencing (NGS) is now widely used in microbiology to explore genome evolution and the structure of pathogen outbreaks. Bioinformatics pipelines readily detect single-nucleotide polymorphisms or short indels. However, bacterial genomes also evolve through the action of small transposable elements called insertion sequences (ISs), which are difficult to detect due to their short length and multiple repetitions throughout the genome. We designed software for the detection of IS insertions in the genomes of prokaryotes. has been released as open source software (GPL3) available from https://github.com/bvalot/panISa. In this study, we assessed the utility of this software for evolutionary studies, by reanalysing five published datasets for outbreaks of human major pathogens in which ISs had not been specifically investigated. We reanalysed the raw data from each study, by aligning the reads against reference genomes and running on the alignments. Each hit was automatically curated and IS-related events were validated on the basis of nucleotide sequence similarity, by comparison with the ISFinder database. In , the pipeline identified IS or IS upstream from the gene, which encodes a cephalosporinase in all third-generation cephalosporin-resistant isolates. In the genomes of isolates, we found that early Haitian isolates had the same ISs as Nepalese isolates, confirming the inferred history of the contamination of this island. In , identified regions of high plasticity, including a pathogenicity island enriched in IS-related events. The overall distribution of ISs deduced with was consistent with SNP-based phylogenic trees, for all species considered. The role of ISs in pathogen evolution has probably been underestimated due to difficulties detecting these transposable elements. We show here that is a useful addition to the bioinformatics toolbox for analyses of the evolution of bacterial genomes. will facilitate explorations of the functional impact of ISs and improve our understanding of prokaryote evolution.