Fiche publication
Date publication
décembre 2015
Journal
Journal of structural biology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DUMAS Dominique
,
Pr GILLET Pierre
,
Pr MAINARD Didier
Tous les auteurs :
Brion A, Zhang G, Dossot M, Moby V, Dumas D, Hupont S, Piet MH, Bianchi A, Mainard D, Galois L, Gillet P, Rousseau M
Lien Pubmed
Résumé
Osteoarthritis (OA) is the most common cause of joint chronic pain and involves the entire joints. Subchondral osteoarthritic osteoblasts present a mineralization defect and, to date, only a few molecules (Vitamin D3 and Bone Morphogenetic Protein2) could improve the mineralization potential of this cell type. In this context, we have tested for the first time the effect of nacre extract on the mineralization capacity of osteoblasts from OA patients. Nacre extract is known to contain osteogenic molecules which have demonstrated their activities notably on the MC3T3 pre-osteoblastic cell line. For this goal, molecules were extracted from nacre (ESM, Ethanol Soluble Matrix) and tested on osteoblasts of the subchondral bone from OA patients undergoing total knee replacement and on MC3T3 cells for comparison. We chose to investigate the mineralization with Alizarin Red staining and with the study of extracellular matrix (ECM) structure and composition. In a complementary way the structure of the ECM secreted during the mineralization phase was investigated using second harmonic generation (SHG). Nacre extract was able to induce the early presence (after 7 days) of precipitated calcium in cells. Raman spectroscopy and electron microscopy showed the presence of nanograins of an early crystalline form of calcium phosphate in OA osteoblasts ECM and hydroxyapatite in MC3T3 ECM. SHG collagen fibers signal was present in both cell types but lower for OA osteoblasts. In conclusion, nacre extract was able to rapidly restore the mineralization capacity of osteoarthritis osteoblasts, therefore confirming the potential of nacre as a source of osteogenic compounds.
Mots clés
3T3 Cells, Animals, Arthroplasty, Replacement, Knee, Calcification, Physiologic, drug effects, Calcium Phosphates, metabolism, Cell Line, Core Binding Factor Alpha 1 Subunit, biosynthesis, Durapatite, metabolism, Extracellular Matrix, metabolism, Humans, Mice, Microscopy, Electron, Scanning, Nacre, pharmacology, Osteoarthritis, metabolism, Osteoblasts, metabolism, Osteocalcin, biosynthesis, Osteopontin, biosynthesis, Real-Time Polymerase Chain Reaction, Spectrum Analysis, Raman
Référence
J. Struct. Biol.. 2015 Dec;192(3):500-9
