Fiche publication
Date publication
janvier 2015
Journal
Methods in cell biology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr GOETZ Jacky
,
Dr CHARVIN Gilles
Tous les auteurs :
Boselli F, Goetz JG, Charvin G, Vermot J
Lien Pubmed
Résumé
Primary cilia are necessary for shear stress sensing in different developing organs such as the kidneys and blood vessels. In endothelial cells (ECs), primary cilia bend in response to blood flow forces and are necessary for flow sensing as well as for the control of angiogenesis. The different parameters guiding cilia bending reflect the forces generated at the surface of the ECs and the mechanical properties of the endothelial cilia. Here, we present an approach allowing the calculation of the bending rigidity of endothelial cilia based on live imaging. The method relies on segmentation and mathematical modeling to extract the critical parameters needed for the calculation.
Mots clés
Animals, Biomechanical Phenomena, physiology, Cell Movement, genetics, Cilia, physiology, Embryo, Nonmammalian, blood supply, Endothelial Cells, cytology, Image Processing, Computer-Assisted, Mechanotransduction, Cellular, physiology, Microscopy, Confocal, Models, Theoretical, Regional Blood Flow, physiology, Stress, Mechanical, Zebrafish, embryology, Zebrafish Proteins, genetics
Référence
Methods Cell Biol.. 2015 ;127:161-73
