Fiche publication
Date publication
mars 2014
Journal
Cell reports
Auteurs
Membres identifiés du Cancéropôle Est :
Dr GOETZ Jacky
,
Dr CHARVIN Gilles
Tous les auteurs :
Goetz JG, Steed E, Ferreira RR, Roth S, Ramspacher C, Boselli F, Charvin G, Liebling M, Wyart C, Schwab Y, Vermot J
Lien Pubmed
Résumé
The pattern of blood flow has long been thought to play a significant role in vascular morphogenesis, yet the flow-sensing mechanism that is involved at early embryonic stages, when flow forces are low, remains unclear. It has been proposed that endothelial cells use primary cilia to sense flow, but this has never been tested in vivo. Here we show, by noninvasive, high-resolution imaging of live zebrafish embryos, that endothelial cilia progressively deflect at the onset of blood flow and that the deflection angle correlates with calcium levels in endothelial cells. We demonstrate that alterations in shear stress, ciliogenesis, or expression of the calcium channel PKD2 impair the endothelial calcium level and both increase and perturb vascular morphogenesis. Altogether, these results demonstrate that endothelial cilia constitute a highly sensitive structure that permits the detection of low shear forces during vascular morphogenesis.
Mots clés
Animals, Cardiovascular System, embryology, Cells, Cultured, Cilia, physiology, Embryo, Nonmammalian, blood supply, Embryonic Development, Endothelial Cells, cytology, Neovascularization, Physiologic, Zebrafish, embryology
Référence
Cell Rep. 2014 Mar;6(5):799-808