Fiche publication
Date publication
juin 2020
Journal
British journal of pharmacology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr LIZARD Gérard
Tous les auteurs :
Nury T, Zarrouk A, Yammine A, Mackrill JJ, Vejux A, Lizard G
Lien Pubmed
Résumé
Oxysterols are oxidized forms of cholesterol generated from cholesterol either by auto-oxidation, enzymatic processes or both. Some of them (7-ketocholesterol, 7β-hydroxycholesterol, 24(S)-hydroxycholesterol), when used at cytotoxic concentrations on different cell types from different species (mesenchymal bone marrow cells, monocytic cells, nerve cells), induce a type of cell death associated with OXIdative stress and several characteristics of APOPTOsis and autoPHAGY, defined as oxiapoptophagy. Oxidative stress is associated with overproduction of reactive oxygen species, increased antioxidant enzyme activities, lipid peroxidation and protein carbonylation. Apoptosis is associated with activation of the mitochondrial pathway: opening of the mitochondrial permeability pore, loss of mitochondrial membrane potential, caspase-3 activation, poly (ADP-ribose) polymerase (PARP) degradation, nuclear condensation and/or fragmentation. Autophagy is characterized by autophagic vacuoles revealed by monodansylcadaverine staining and transmission electron microscopy, and an increased ratio (LC-3II/LC-3I). In addition, morphological, topographical and functional changes of the peroxisome are observed.
Mots clés
7-ketocholesterol; 7β-hydroxycholesterol; 24(S)-hydroxycholesterol, cytoprotection, oxiapoptophagy, oxysterols
Référence
Br. J. Pharmacol.. 2020 Jun 24;:
