Direct conjugation of antibodies to the ZnS shell of quantum dots for FRET immunoassays with low picomolar detection limits.

Date publication

décembre 2016

Journal

Chemical communications (Cambridge, England)

Auteurs

Membres identifiés du Cancéropôle Est :
Dr CHARBONNIERE Loïc

Tous les auteurs :
Bhuckory S, Mattera L, Wegner KD, Qiu X, Wu YT, Charbonnière LJ, Reiss P, Hildebrandt N

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/27901526

Résumé

Compact and functional nanoparticle-antibody conjugates are of paramount importance for the development of quantum dot (QD)-based immunoassays. Here, we present a simple strategy to directly conjugate IgG, F(ab'), and Fab antibodies via their endogenous disulfide groups directly to the inorganic ZnS shell of compact penicillamine-coated QDs. The functionality of the conjugates was demonstrated by terbium (Tb)-to-QD FRET immunoassays against prostate specific antigen in serum samples. Detection limits of 2.5 pM (0.080 ng mL) were 10 and 25 times lower compared to conjugation via maleimide-terminated ligands and polymer chains, respectively. These more compact, simple, and sensitive QD-antibody conjugates will be highly advantageous for nanocrystal-based biosensing applications.

Référence

Chem. Commun. (Camb.). 2016 Dec 13;52(100):14423-14425