Mono- and Poly-unsaturated Phosphatidic Acid Regulate Distinct Steps of Regulated Exocytosis in Neuroendocrine Cells.

Fiche publication

Date publication

août 2020

Journal

Cell reports

Auteurs

Membres identifiés du Cancéropôle Est :
Dr BADER Marie-France , Dr GASMAN Stéphane , Dr CHASSEROT-GOLAZ Sylvette , Dr VITALE Nicolas , Dr ORY Stéphane

Tous les auteurs :
Tanguy E, Costé de Bagneaux P, Kassas N, Ammar MR, Wang Q, Haeberlé AM, Raherindratsara J, Fouillen L, Renard PY, Montero-Hadjadje M, Chasserot-Golaz S, Ory S, Gasman S, Bader MF, Vitale N

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/32814056

Résumé

Specific forms of fatty acids are well known to have beneficial health effects, but their precise mechanism of action remains elusive. Phosphatidic acid (PA) produced by phospholipase D1 (PLD1) regulates the sequential stages underlying secretory granule exocytosis in neuroendocrine chromaffin cells, as revealed by pharmacological approaches and genetic mouse models. Lipidomic analysis shows that secretory granule and plasma membranes display distinct and specific composition in PA. Secretagogue-evoked stimulation triggers the selective production of several PA species at the plasma membrane near the sites of active exocytosis. Rescue experiments in cells depleted of PLD1 activity reveal that mono-unsaturated PA restores the number of exocytotic events, possibly by contributing to granule docking, whereas poly-unsaturated PA regulates fusion pore stability and expansion. Altogether, this work provides insight into the roles that subspecies of the same phospholipid may play based on their fatty acyl chain composition.