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Date publication
septembre 2020
Journal
Microorganisms
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GANGLOFF Sophie
Tous les auteurs :
Mongaret C, Varin-Simon J, Lamret F, El-Mahdy TS, Brasme L, Vernet-Garnier V, Gangloff SC, Ohl X, Reffuveille F
Lien Pubmed
Résumé
is an opportunistic pathogen involved in Bone and Prosthesis Infections (BPIs). In this study, we observed the behavior of commensal and BPI strains in the bone environment through bacterial internalization by osteoblast-like cells and biofilm formation. For the commensal strains, less than 1% of the bacteria were internalized; among them, about 32.7 ± 3.9% persisted intracellularly for up to 48 h. infection seems to have no cytotoxic effect on bone cells as detected by LDH assay. Interestingly, commensal showed a significant increase in biofilm formation after osteoblast-like internalization for 50% of the strains (2.8-fold increase). This phenomenon is exacerbated on a titanium support, a material used for medical devices. For the BPI clinical strains, we did not notice any increase in biofilm formation after internalization despite a similar internalization rate by the osteoblast-like cells. Furthermore, fluorescent staining revealed more live bacteria within the biofilm after osteoblast-like cell interaction, for all strains (BPIs and commensal). The genomic study did not reveal any link between their clinical origin and phylotype. In conclusion, we have shown for the first time the possible influence of internalization by osteoblast-like cells on commensal
Mots clés
Cutibacterium acnes, biofilms, host–pathogen interactions, internalization, joint infections
Référence
Microorganisms. 2020 Sep 12;8(9):
