Measurements of Compensatory Endocytosis by Antibody Internalization and Quantification of Endocytic Vesicle Distribution in Adrenal Chromaffin Cells.

Date publication

janvier 2021

Journal

Methods in molecular biology (Clifton, N.J.)

Auteurs

Membres identifiés du Cancéropôle Est :
Dr GASMAN Stéphane , Dr CHASSEROT-GOLAZ Sylvette , Dr VITALE Nicolas , Dr ORY Stéphane

Tous les auteurs :
Ceridono M, Chasserot-Golaz S, Vitale N, Gasman S, Ory S

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/33222126

Résumé

Plasma membrane proteins are amenable to endocytosis assays since they are easily labeled by reagents applied in the extracellular medium. This has been widely exploited to study constitutive endocytosis or ligand-induced receptor endocytosis. Compensatory endocytosis is the mechanism by which components of secretory vesicles are retrieved after vesicle fusion with the plasma membrane in response to cell stimulation and a rise in intracellular calcium. Luminal membrane proteins from secretory vesicles are therefore transiently exposed at the plasma membrane. Here, we described an antibody-based method to monitor compensatory endocytosis in chromaffin cells and present an image-based analysis to quantify endocytic vesicles distribution.

Mots clés

Chromaffin cells, Compensatory endocytosis, Dopamine-β-hydroxylase, Internalization, Large dense core vesicle, Neuroendocrine cells

Référence

Methods Mol Biol. 2021 ;2233:43-51