Dispersibility-Dependent Biodegradation of Graphene Oxide by Myeloperoxidase.

Fiche publication

Date publication

août 2015

Journal

Small (Weinheim an der Bergstrasse, Germany)

Auteurs

Membres identifiés du Cancéropôle Est :
Dr MENARD-MOYON Cécilia , Dr BIANCO Alberto

Tous les auteurs :
Kurapati R, Russier J, Squillaci MA, Treossi E, Ménard-Moyon C, Del Rio-Castillo AE, Vazquez E, Samorì P, Palermo V, Bianco A

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/25959808

Résumé

Understanding human health risk associated with the rapidly emerging graphene-based nanomaterials represents a great challenge because of the diversity of applications and the wide range of possible ways of exposure to this type of materials. Herein, the biodegradation of graphene oxide (GO) sheets is reported by using myeloperoxidase (hMPO) derived from human neutrophils in the presence of a low concentration of hydrogen peroxide. The degradation capability of the enzyme on three different GO samples containing different degree of oxidation on their graphenic lattice, leading to a variable dispersibility in aqueous media is compared. hMPO fails in degrading the most aggregated GO, but succeeds to completely metabolize highly dispersed GO samples. The spectroscopy and microscopy analyses provide unambiguous evidence for the key roles played by hydrophilicity, negative surface charge, and colloidal stability of the aqueous GO in their biodegradation by hMPO catalysis.