Fiche publication
Date publication
décembre 2020
Journal
microPublication biology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr JARRIAULT Sophie
Tous les auteurs :
Ahier A, Suman SK, Jarriault S
Lien Pubmed
Résumé
Strong loss-of-function or null mutants can sometimes lead to a penetrant early lethality, impairing the study of these genes' function. This is the case for the null mutant, which exhibits 100% penetrant lethality. Here, we describe how we used gene bashing to identify distinct regulatory regions in the locus. This allowed us to generate a null strain that is viable and still displays mutant Y-to-PDA transdifferentiation phenotype. Such strategy can be applied to many other mutants impacting viability.
Référence
MicroPubl Biol. 2020 Dec 21;2020:
