Mapping rRNA 2'-O-methylations and identification of C/D snoRNAs in Arabidopsis thaliana plants.

Fiche publication

Date publication

février 2021

Journal

RNA biology

Auteurs

Membres identifiés du Cancéropôle Est :
Pr MOTORINE Iouri , Dr MARCHAND Virginie , Dr CARAPITO Christine

Tous les auteurs :
Azevedo-Favory J, Gaspin C, Ayadi L, Montacié C, Marchand V, Jobet E, Rompais M, Carapito C, Motorin Y, Sáez-Vásquez J

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/33596769

Résumé

In all eukaryotic cells, the most abundant modification of ribosomal RNA (rRNA) is methylation at the ribose moiety (2'--methylation). Ribose methylation at specific rRNA sites is guided by small nucleolar RNAs (snoRNAs) of C/D-box type (C/D snoRNA) and achieved by the methyltransferase Fibrillarin (FIB). Here we used the Illumina-based RiboMethSeq approach for mapping rRNA 2'--methylation sites in Col-0 (WT) plants. This analysis detected novel C/D snoRNA-guided rRNA 2'--methylation positions and also some orphan sites without a matching C/D snoRNA. Furthermore, immunoprecipitation of Arabidopsis FIB2 identified and demonstrated expression of C/D snoRNAs corresponding to majority of mapped rRNA sites. On the other hand, we show that disruption of Arabidopsis Nucleolin 1 gene (NUC1), encoding a major nucleolar protein, decreases 2'--methylation at specific rRNA sites suggesting functional/structural interconnections of 2'--methylation with nucleolus organization and plant development. Finally, based on our findings and existent database sets, we introduce a new nomenclature system for C/D snoRNA in Arabidopsis plants.