Fiche publication
Date publication
janvier 2020
Journal
Methods in enzymology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr GOETZ Jacky
,
Dr HYENNE Vincent
Tous les auteurs :
Mary B, Ghoroghi S, Hyenne V, Goetz JG
Lien Pubmed
Résumé
Formerly considered as insignificant cell debris, extracellular vesicles (EVs) have emerged as potent mediators of cell-cell communication, both in proximity and at distance from the producing cell. EVs are transported in body fluids and can be internalized by specific distant cells to ultimately deliver a functional message. Despite their striking importance in many physiological and pathological contexts, the exact mechanisms by which EVs impose local and distant modifications of the microenvironment in vivo remain to be fully understood. We realized that some conceptual gaps are direct consequences of the difficulty to visualize the shuttling and targeting of EVs in real time in vivo. The zebrafish larvae offered attractive features for live tracking of EVs, within circulating fluids. Here, we describe the experimental procedures that we have built for dissecting the dissemination of EVs at high spatio-temporal resolution in vivo.
Mots clés
Extracellular vesicles, Hemodynamics, Metastasis, Zebrafish
Référence
Methods Enzymol. 2020 ;645:243-275
