Fiche publication
Date publication
juin 2015
Journal
ACS chemical biology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MELY Yves
,
Dr KLYMCHENKO Andrey
Tous les auteurs :
Kreder R, Pyrshev KA, Darwich Z, Kucherak OA, Mély Y, Klymchenko AS
Lien Pubmed
Résumé
Detecting and imaging lipid microdomains (rafts) in cell membranes remain a challenge despite intensive research in the field. Two types of fluorescent probes are used for this purpose: one specifically labels a given phase (liquid ordered, Lo, or liquid disordered, Ld), while the other, being environment-sensitive (solvatochromic), stains the two phases in different emission colors. Here, we combined the two approaches by designing a phase-sensitive probe of the Ld phase and a quencher of the Ld phase. The former is an analogue of the recently developed Nile Red-based probe NR12S, bearing a bulky hydrophobic chain (bNR10S), while the latter is based on Black Hole Quencher-2 designed as bNR10S (bQ10S). Fluorescence spectroscopy of large unilamellar vesicles and microscopy of giant vesicles showed that the bNR10S probe can partition specifically into the Ld phase, while bQ10S can specifically quench the NR12S probe in the Ld phase so that only its fraction in the Lo phase remains fluorescent. Thus, the toolkit of two probes with quencher can specifically target Ld and Lo phases and identify their lipid order from the emission color. Application of this toolkit in living cells (HeLa, CHO, and 293T cell lines) revealed heterogeneity in the cell plasma membranes, observed as distinct probe environments close to the Lo and Ld phases of model membranes. In HeLa cells undergoing apoptosis, our toolkit showed the formation of separate domains of the Ld-like phase in the form of blebs. The developed tools open new possibilities in lipid raft research.
Mots clés
Animals, Apoptosis, CHO Cells, Cell Survival, Cricetulus, Fluorescence Resonance Energy Transfer, Fluorescent Dyes, chemical synthesis, HEK293 Cells, HeLa Cells, Humans, Hydrophobic and Hydrophilic Interactions, Membrane Microdomains, metabolism, Oxazines, chemical synthesis, Phase Transition, Unilamellar Liposomes, chemistry
Référence
ACS Chem. Biol.. 2015 Jun;10(6):1435-42
