Characterization and biodistribution of Au nanoparticles loaded in PLGA nanocarriers using an original encapsulation process.

Fiche publication

Date publication

mai 2021

Journal

Colloids and surfaces. B, Biointerfaces

Auteurs

Membres identifiés du Cancéropôle Est :
Pr DENAT Franck , Pr ROUX Stéphane , Pr BEDUNEAU Arnaud

Tous les auteurs :
Laurent G, Benbalit C, Chrétien C, Dupuis C, Pellequer Y, Bazzi R, Thakare VS, Denat F, Roux S, Béduneau A

Lien Pubmed

https://www.ncbi.nlm.nih.gov/pubmed/34058691

Résumé

Due to their imaging and radiosensitizing properties, ultrasmall gadolinium chelate-coated gold nanoparticles (AuNP) represent a promising approach in the diagnosis and the treatment of tumors. However, their poor pharmacokinetic profile, especially their rapid renal clearance prevents from an efficient exploitation of their potential for medical applications. The present study focuses on a strategy which resides in the encapsulation of AuNP in large polymeric NP to avoid the glomerular filtration and then to prolong the vascular residence time. An original encapsulation procedure using the polyethyleneimine (PEI) was set up to electrostatically entrap AuNP in biodegradable poly(lactic-co-glycolic acid) (PLGA) and polyethylene glycol -PLGA (PLGA-PEG) NP. Hydrodynamic diameters of NP were dependent of the PEI/Au ratio and comprised between 115 and 196 nm for ratios equal or superior to 4. Encapsulation yield was close to 90 % whereas no loading was observed without PEI. No toxicity was observed after 24 h exposure in hepatocyte cell-lines. Entrapement of AuNP in polymeric nanocarriers facilitated the passive uptake in cancer cells after only 2 h incubation. In healthy rat, the encapsulation allowed increasing the gold concentration in the blood within the first hour after intravenous administration. Polymeric nanoparticles were sequestered in the liver and the spleen rather than the kidneys. T-weighted magnetic resonance demonstrated that encapsulation process did not alter the contrast agent properties of gadolinium. The encapsulation of the gold nanoparticles in PLGA particles paves the way to innovative imaging-guided anticancer therapies in personalized medicine.